| Description | This product is a human monoclonal antibody provided by Creative Biolabs. The antibody is capable of recognizing human CD40 Molecule. It can be used for CD40 detection in Enzyme-Linked Immunosorbent Assay (ELISA), Flow Cytometry (FC) and as an Agonist. The antibody is expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultra purified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Immunogen | Soluble human CD40 |
| Isotype | IgG2 kappa |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form. |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A or G purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; FC; Agonist |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Anti-Human CD40 Agonist Monoclonal Antibody (V3S-0822-YC3126) in SDS-PAGE
SDS-PAGE analysis of V3S-0822-YC3126 in non-reduced conditions (Lane 1) and reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as about 150-245 kDa.
Figure 1 Anti-Human CD40 Agonist Monoclonal Antibody (V3S-0822-YC3126) in SDS-PAGE
Figure 2 Anti-Human CD40 Agonist Monoclonal Antibody (V3S-0822-YC3126) in SEC-HPLC
The purity of V3S-0822-YC3126 was greater than 95% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
Figure 2 Anti-Human CD40 Agonist Monoclonal Antibody (V3S-0822-YC3126) in SEC-HPLC
Figure 3 Anti-Human CD40 Agonist Monoclonal Antibody (V3S-0822-YC3126) in ELISA
ELISA analysis of V3S-0822-YC3126 was performed by coating with Recombinant Human CD40 Protein. Then blocked with BSA, and incubated with Anti-Human CD40 Antibody (V3S-0822-YC3126). The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 3 Anti-Human CD40 Agonist Monoclonal Antibody (V3S-0822-YC3126) in ELISA
