| Description | This product is a human monoclonal antibody that specifically binds to human decay accelerating factor (DAF, CD55). The antibody cross-reacted strongly with the lung adenocarcinoma line, A549. The mAb also may be used to assess DAF overexpression by IHC assay. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Affinity | 13 nM |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | FC; ELISA; IHC |
| Application Notes | Biacore CM-5 chips were functionalized with 350 response units recombinant human DAF in 10 mM sodium acetate (pH 4.6) or 8,000 response units bovine serum albumin as a negative control. The DAF-derivatized chip was saturated with LU30 scFv fragment (25-100 nM) by injecting this fragment at 10 μl/min in PBS containing 0.5 % bovine serum albumin and 0.05 % TWEEN 20™. The resultant sensorgrams were analyzed. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
