| Description | This is a mouse monoclonal antibody that specifically targets the human CD58 protein. The antibody has demonstrated significant inhibition of both xenogeneic and allogeneic mixed lymphocyte reactions (MLR), achieving inhibition rates ranging from 80% to 100%. The immunogen utilized in the development of this antibody comprises HT16/CHO cells, specifically CHO cells transfected with BG8. BG8 is an animal cell expression vector that encodes transmembrane LFA-3. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Immunogen | HT16/CHO and the CHO cells transfected with BG8 (an animal cell expression vector encoding transmembrane LFA-3). |
| Isotype | Mouse IgG1 |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein G affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | Inhib; FC |
| Application Notes | Inhib: 10 µg/mL FC: 10 µg/mL |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | CD58 |
| Alternative Name | CD58 Molecule; CD58 Antigen, (Lymphocyte Function-Associated Antigen 3); Surface Glycoprotein LFA-3; LFA3; AG3; Lymphocyte Function-Associated Antigen 3; CD58 Antigen; LFA-3 |
| Gene ID | 965 |
| UniProt | P19256 |
| Related pathway | Innate Immune System and Response to elevated platelet cytosolic Ca2+ |
| Research Area | Immunology; Signal Pathway |
| Related Disease | Acute and chronic inflammation, autoimmune diseases, and for immunomodulation, including such diseases as systemic lupus erythematosus or lupus vulgaris, rheumatoid arthritis and thyroiditis |
