Recombinant Anti-CD83 Antibody (V3S-0522-YC5434) (CAT#: V3S-0522-YC5434)

Figure 1 Recombinant Anti-CD83 Antibody (V3S-0522-YC5434) in Western Blot.

Western blot analysis of V3S-0522-YC5434 was performed by loading recombinant human CD83 protein.

TAB-353LC incubation concentration: 2 ng/μL.
The secondary antibody: HRP-goat anti-human IgG
Lane 1: Reduced antigen (0.6 μg)

Figure 2 Recombinant Anti-CD83 Antibody (V3S-0522-YC5434) in ELISA.

ELISA analysis of V3S-0522-YC5434 was performed by coating with recombinant human CD83 protein. Then blocked with BSA, and incubated with human anti-CD83 recombinant Antibody (V3S-0522-YC5434). The goat anti-human IgG-HRP as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 3 Recombinant Anti-CD83 Antibody (V3S-0522-YC5434) Fab Fragment in Western Blot.

Western blot analysis of V3S-0522-YC5434-Fab was performed by loading recombinant human CD83 protein.

V3S-0522-YC5434-Fab incubation concentration: 2 ng/μL.
The secondary antibody: HRP-goat anti-human IgG
Lane 1: Reduced antigen (0.6 μg)

Figure 4 Recombinant Anti-CD83 Antibody (V3S-0522-YC5434) Fab Fragment in ELISA.

ELISA analysis of V3S-0522-YC5434-Fab was performed by coating with recombinant human CD83 protein. Then blocked with BSA, and incubated with human anti-CD83 recombinant Antibody Fab fragment (V3S-0522-YC5434-Fab). The goat anti-human IgG-HRP as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.