Recombinant Anti-Cd8a Antibody (V3S-0822-YC334) (CAT#: V3S-0822-YC334)

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Datasheet

MSDS

COA

Summary
Property
Applications
Protocols
Target

Summary

Description This product is a rat monoclonal antibody provided by Creative Biolabs. The monoclonal antibody is capable of recognizing mouse CD8 antigen, alpha chain. It can be used for Cd8a detection in Immunohistochemistry-Frozen (IHC-Fr) and Depletion Assay (Dep). The antibody is expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultra purified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg.
Clonality Monoclonal
Host Species Rat
Target Species Mouse
Immunogen CBA mouse thymocytes
Isotype IgG2b

Property

Purity >95%, determined by SDS-PAGE and SEC-HPLC
Purification Affinity Chromatography Purified
Formulation 0.01 M PBS, pH 7.2, 150 mM NaCl
Preservation No preservatives
Storage Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics.

Applications

Application IHC-Fr, Dep

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target Cd8a
Alternative Name Ly-2; Ly-B; Ly-35; Lyt-2; BB154331
Gene ID 12525
UniProt P01731
Research Area Immunology
For research use only, not directly for clinical use.
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