| Description | This product is a recombinant Human antibody provided by CreativeBiolabs. The antibody is specific for Carcinoembryonic Antigen Related Cell Adhesion Molecule 6. It can be used for Carcinoembryonic Antigen Related Cell Adhesion Molecule 6 detection in Western Blot, Enzyme-Linked Immunosorbent Assay and Flow Cytometry. It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultrapurified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human, Cynomolgus |
| Immunogen | Cynomolgus CEACAM6 N-terminal domain 1 |
| Epitope | In the N-terminal domain 1. |
| Affinity | The affinity is in nanomolar range, about 3.7 nM, determined by surface plasmon resonance. |
| Isotype | IgG2 kappa |
| Expression Species | HEK293F or CHO Cell line |
| Conjugation | Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form. |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Purified with Protein A or G affinity chromatography |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | DB; WB; ELISA; FC |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | CEACAM6 |
| Alternative Name | Carcinoembryonic Antigen-Related Cell Adhesion Molecule 6 (Non-Specific Cross Reacting Antigen); Normal Cross-Reacting Antigen; NCA; Non-Specific Crossreacting Antigen; Cluster Of Differentiation 66c; CD66c Antigen; CD66c; CEAL |
| Research Area | Cell Biology |
| Related Disease | Cancer |
Figure 1 Mouse Anti-CEACAM6 Monoclonal Antibody (V3S-1022-YC2559) in ELISA
ELISA analysis of V3S-1022-YC2559 was performed by coating with human CEACAM6 protein (His tag). Then blocked with BSA and incubated with V3S-1022-YC2559. The HRP-conjugated goat anti-IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 1 Mouse Anti-CEACAM6 Monoclonal Antibody (V3S-1022-YC2559) in ELISA
Figure 2 Mouse Anti-CEACAM6 Monoclonal Antibody (V3S-1022-YC2559) in WB
Western blot analysis of V3S-1022-YC2559 was performed by loading human CEACAM6 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-1022-YC2559 and HRP goat anti-IgG (H+L) as a secondary antibody (1: 3000). Chemiluminescent detection was performed.
Figure 2 Mouse Anti-CEACAM6 Monoclonal Antibody (V3S-1022-YC2559) in WB
Figure 3 Mouse Anti-CEACAM6 Monoclonal Antibody (V3S-1022-YC2559) in DB
Dot Blot analysis of V3S-1022-YC2559 was performed by coating with human CEACAM6 protein (His tag).
V3S-1022-YC2559 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-IgG (H+L) as a secondary antibody (1: 3000)
NC: Negative control, PC: Positive control
Figure 3 Mouse Anti-CEACAM6 Monoclonal Antibody (V3S-1022-YC2559) in DB
