| Description | This product is an anti-complement factor H (CFH) antibody. The mAb is an inhibitor of CFH. The antibody binds to an epitope within peptide GPPPPIDNGDITSFP of CFH protein. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Epitope | Peptide GPPPPIDNGDITSFP |
| Affinity | 2.5 pM |
| Isotype | IgG3 |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; DB; FuncS |
| Application Notes | The binding affinity of the antibody was measured at 25, 50, or 100 nM against the immobilized peptide of GPPPPIDNGDITSFP using SPR in a BIAcore instrument. This analysis revealed an off-rate (kd) of 5.56×10-7 s−1, an on-rate (ka) of 2.26×105 M−1 s−1 and an affinity (KD) of 2.46 nM. Complement-Dependent Cytotoxicity (CDC) assay A549 lung cancer cells were incubated with CFH antibody 7968 along with normal human serum (NHS) as a source of complement. The antibody was tested at 60 or 120 μg/mL and measured cytotoxicity by an LDH-release ELISA. The results showed that mAb 7968 caused a statistically significant increase in CDC at 60 μg/mL and 120 μg/mL. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | CFH |
| Alternative Name | FH; HF; HF1; HF2; HUS; FHL1; AHUS1; AMBP1; ARMD4; ARMS1; CFHL3; complement factor H; H factor 1 (complement); H factor 2 (complement); adrenomedullin binding protein; age-related maculopathy susceptibility 1; beta-1-H-globulin; beta-1H; complement factor H, isoform b; factor H; factor H-like 1 |
| Gene ID | 3075 |
| UniProt | P08603 |
| Research Area | Immunology |
