Recombinant Anti-CFH Neutralizing Antibody (V3S-0622-YC104) (CAT#: V3S-0622-YC104)

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Datasheet

MSDS

COA

Summary
Property
Applications
Protocols
Target

Summary

Description This product is an anti-complement factor H (CFH) antibody. The mAb is an inhibitor of CFH. The antibody binds to an epitope within peptide GPPPPIDNGDITSFP of CFH protein.
Clonality Monoclonal
Host Species Human
Target Species Human
Epitope Peptide GPPPPIDNGDITSFP
Affinity 2.5 pM
Isotype IgG3

Property

Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and SEC-HPLC
Endotoxin <1 EU/mg
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4°C within a week. For longer storage, aliquot and store at -20°C.

Applications

Application ELISA; DB; FuncS
Application Notes The binding affinity of the antibody was measured at 25, 50, or 100 nM against the immobilized peptide of GPPPPIDNGDITSFP using SPR in a BIAcore instrument. This analysis revealed an off-rate (kd) of 5.56×10-7 s−1, an on-rate (ka) of 2.26×105 M−1 s−1 and an affinity (KD) of 2.46 nM.
Complement-Dependent Cytotoxicity (CDC) assay
A549 lung cancer cells were incubated with CFH antibody 7968 along with normal human serum (NHS) as a source of complement. The antibody was tested at 60 or 120 μg/mL and measured cytotoxicity by an LDH-release ELISA. The results showed that mAb 7968 caused a statistically significant increase in CDC at 60 μg/mL and 120 μg/mL.

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target CFH
Alternative Name FH; HF; HF1; HF2; HUS; FHL1; AHUS1; AMBP1; ARMD4; ARMS1; CFHL3; complement factor H; H factor 1 (complement); H factor 2 (complement); adrenomedullin binding protein; age-related maculopathy susceptibility 1; beta-1-H-globulin; beta-1H; complement factor H, isoform b; factor H; factor H-like 1
Gene ID 3075
UniProt P08603
Research Area Immunology
For research use only, not directly for clinical use.
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