| Description | This product is a monoclonal antibody developed against the CIN0327 strain. The antibody binds to G-protein at the antigenic site 122-136, a part of the B-cell epitope amino acids 122 to 158. NAbC also showed its ability to neutralize CHPV strains 1653514, 1015405, 12588, 1210586 and 148974. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Chandipura virus (CHPV) |
| Cross Reactivity | Andhra pradesh/Telangana (2003 strain CIN0327), Strain I653514, Gujarat (2014 strain 148974) |
| Immunogen | Indian CHPV strain (CIN0327) |
| Epitope | A conserved linear epitope: SGTLVSPGFPPESCG (AA 122-136) |
| ND50 | MAb (NAbC) neutralized 100TCID₅₀ virus with ND₅₀ titer>12800 |
| Isotype | Mouse IgG2a, κ |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein G purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | Neut; ELISA; WB |
| Application Notes | Neutralization assay: NAbC at dilution 1/12800 to neutralize strain CIN0327. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | CHPV G-protein |
| Alternative Name | Chandipura virus G-protein; G-protein; Chandipura virus; CHPV |
| Research Area | Neuroscience |
