| Description | This product is a human monoclonal antibody that reacts with CSF2RA. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Immunogen | Human GM-CSF Receptor alpha transfected COS cells |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | IF; IP; Neut; FuncS; ELISA; FC; WB |
| Application Notes | The antibody is recommended for detection of CSF2RA by IF, IP, Neut, FuncS, ELISA, FC, WB assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | CSF2RA |
| Alternative Name | CSF2RA; colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage); CSF2R; granulocyte-macrophage colony-stimulating factor receptor subunit alpha; CD116; GMR-alpha; GMCSFR-alpha; CD116 antigen; GM-CSF re |
| Gene ID | 1438 |
| UniProt | P15509 |
| Research Area | Immunology |
Figure 1 Anti-Human CSF2RA Antibody (V3S-0622-YC1423) in ELISA.
ELISA analysis of V3S-0622-YC1423 was performed by coating with recombinant human GM-CSF Receptor alpha (CSF2RA) protein (his Tag).
The secondary antibody: HRP-goat anti-human IgG (H+L)
Figure 1 Anti-Human CSF2RA Antibody (V3S-0622-YC1423) in ELISA.
Figure 2 Anti-Human CSF2RA Antibody (V3S-0622-YC1423) in Western Blot.
Western blot analysis of V3S-0622-YC1423 was performed by loading recombinant human GM-CSF Receptor alpha (CSF2RA) protein (his Tag).
V3S-0622-YC1423 incubation concentration: 2ng/μL.
The secondary antibody: HRP-goat anti-human IgG (H+L)
Lane 1: Reduced antigen (0.1μg)
Lane 2: Reduced antigen (0.5μg)
Lane 3: Reduced antigen (1μg)
Figure 2 Anti-Human CSF2RA Antibody (V3S-0622-YC1423) in Western Blot.
Figure 3 Anti-Human CSF2RA Antibody (V3S-0622-YC1423) in Dot Blot.
Dot blot analysis of V3S-0622-YC1423 was performed by coating with recombinant human GM-CSF Receptor alpha (CSF2RA) protein (his Tag).
V3S-0622-YC1423 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-goat anti-human IgG (H+L)
Figure 3 Anti-Human CSF2RA Antibody (V3S-0622-YC1423) in Dot Blot.
