Anti-CTGF Neutralizing Antibody (V3S-0522-YC1469) (CAT#: V3S-0522-YC1469)

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  • fig1
    Figure 1 Recombinant Anti-CTGF Monoclonal Antibody (V3S-0522-YC1469) in SDS-PAGE.
  • fig1
    Figure 2 Recombinant Anti-CTGF Monoclonal Antibody (V3S-0522-YC1469) in SEC-HPLC.
  • fig1
    Figure 3 Recombinant Anti-CTGF Monoclonal Antibody (V3S-0522-YC1469) in WB.
  • fig1
    Figure 4 Recombinant Anti-CTGF Monoclonal Antibody (V3S-0522-YC1469) in DB.
  • fig1
    Figure 5 Recombinant Anti-CTGF Monoclonal Antibody (V3S-0522-YC1469) in ELISA.

Datasheet

MSDS

COA

Summary
Property
Applications
Protocols
Target

Summary

Description This product is a monoclonal antibody derived from Human (Homo sapiens), which can specifically recognize connective tissue growth factor. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation.
Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality Monoclonal
Host Species Human
Target Species Human
Isotype IgG1, κ

Property

Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.

Applications

Application WB; DB; ELISA; IHC; FC; IP; IF; Inhib
Application Notes The antibody is recommended for detection of CTGF by ELISA, IHC, FC, IP, IF, Inhib assays.

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target CTGF
Alternative Name CTGF; connective tissue growth factor; CCN2; NOV2; HCS24; IGFBP8; IBP-8; IGFBP-8; CCN family member 2; IGF-binding protein 8; hypertrophic chondrocyte-specific protein 24; insulin-like growth factor-binding protein 8; FG-3019; FG3019
Gene ID 1490
UniProt P29279
Research Area Cardiovascular
Related Disease Duchenne muscular dystrophy, Hepatic fibrosis, Idiopathic pulmonary fibrosis, Pancreatic cancer

Tested Data

SDS-PAGE

Figure 1 Recombinant Anti-CTGF Monoclonal Antibody (V3S-0522-YC1469) in SDS-PAGE.

SDS-PAGE analysis of V3S-0522-YC1469 in β-mercaptoethanol-reduced conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.

Figure 1 Recombinant Anti-CTGF Monoclonal Antibody (V3S-0522-YC1469) in SDS-PAGE.

SEC-HPLC

Figure 2 Recombinant Anti-CTGF Monoclonal Antibody (V3S-0522-YC1469) in SEC-HPLC.

The purity of V3S-0522-YC1469 was greater than 95% as determined by SEC-HPLC.

Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl

Figure 2 Recombinant Anti-CTGF Monoclonal Antibody (V3S-0522-YC1469) in SEC-HPLC.

WB

Figure 3 Recombinant Anti-CTGF Monoclonal Antibody (V3S-0522-YC1469) in WB.

Western blot analysis was performed by loading 0.1 µg (lane 1), 0.3 µg(lane 2), and 0.6 µg (lane 3) recombinant human CTGF onto a 12% Tris-HCl polyacrylamide gel.

Figure 3 Recombinant Anti-CTGF Monoclonal Antibody (V3S-0522-YC1469) in WB.

DB

Figure 4 Recombinant Anti-CTGF Monoclonal Antibody (V3S-0522-YC1469) in DB.

Dot Blot analysis of V3S-0522-YC1469 was performed by coating with human CTGF antigen.

Figure 4 Recombinant Anti-CTGF Monoclonal Antibody (V3S-0522-YC1469) in DB.

ELISA

Figure 5 Recombinant Anti-CTGF Monoclonal Antibody (V3S-0522-YC1469) in ELISA.

ELISA analysis of V3S-0522-YC1469 was performed by coating with Human CTGF protein, His Tag.

Figure 5 Recombinant Anti-CTGF Monoclonal Antibody (V3S-0522-YC1469) in ELISA.

For research use only, not directly for clinical use.
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