Description | This product is a monoclonal antibody derived from Human (Homo sapiens), which can specifically recognize connective tissue growth factor. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
Clonality | Monoclonal |
Host Species | Human |
Target Species | Human |
Isotype | IgG1, κ |
Expression Species | HEK293F or CHO |
Conjugation | None |
Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
Endotoxin | <1 EU/mg, determined by LAL method |
Purification | Protein A affinity purified |
Sterility | 0.2 μM filtered |
Formulation | PBS, pH 7.4 |
Preservation | No preservatives |
Stabilizer | No stabilizers |
Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
Application | WB; DB; ELISA; IHC; FC; IP; IF; Inhib |
Application Notes | The antibody is recommended for detection of CTGF by ELISA, IHC, FC, IP, IF, Inhib assays. |
ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
WB | Western Blot Protocol |
FC | Flow Cytometry Protocol |
Target | CTGF |
Alternative Name | CTGF; connective tissue growth factor; CCN2; NOV2; HCS24; IGFBP8; IBP-8; IGFBP-8; CCN family member 2; IGF-binding protein 8; hypertrophic chondrocyte-specific protein 24; insulin-like growth factor-binding protein 8; FG-3019; FG3019 |
Gene ID | 1490 |
UniProt | P29279 |
Research Area | Cardiovascular |
Related Disease | Duchenne muscular dystrophy, Hepatic fibrosis, Idiopathic pulmonary fibrosis, Pancreatic cancer |
Figure 1 Recombinant Anti-CTGF Monoclonal Antibody (V3S-0522-YC1469) in SDS-PAGE.
SDS-PAGE analysis of V3S-0522-YC1469 in β-mercaptoethanol-reduced conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
Figure 1 Recombinant Anti-CTGF Monoclonal Antibody (V3S-0522-YC1469) in SDS-PAGE.
Figure 2 Recombinant Anti-CTGF Monoclonal Antibody (V3S-0522-YC1469) in SEC-HPLC.
The purity of V3S-0522-YC1469 was greater than 95% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
Figure 2 Recombinant Anti-CTGF Monoclonal Antibody (V3S-0522-YC1469) in SEC-HPLC.
Figure 3 Recombinant Anti-CTGF Monoclonal Antibody (V3S-0522-YC1469) in WB.
Western blot analysis was performed by loading 0.1 µg (lane 1), 0.3 µg(lane 2), and 0.6 µg (lane 3) recombinant human CTGF onto a 12% Tris-HCl polyacrylamide gel.
Figure 3 Recombinant Anti-CTGF Monoclonal Antibody (V3S-0522-YC1469) in WB.
Figure 4 Recombinant Anti-CTGF Monoclonal Antibody (V3S-0522-YC1469) in DB.
Dot Blot analysis of V3S-0522-YC1469 was performed by coating with human CTGF antigen.
Figure 4 Recombinant Anti-CTGF Monoclonal Antibody (V3S-0522-YC1469) in DB.
Figure 5 Recombinant Anti-CTGF Monoclonal Antibody (V3S-0522-YC1469) in ELISA.
ELISA analysis of V3S-0522-YC1469 was performed by coating with Human CTGF protein, His Tag.
Figure 5 Recombinant Anti-CTGF Monoclonal Antibody (V3S-0522-YC1469) in ELISA.