| Sub Cat | Target Species | Epitope | Isotype | Application | Affinity | |
|---|---|---|---|---|---|---|
| V3S-1124-YC620 | Human, Mouse | IgG1 | WB; ELISA; Neut; FC | |||
| V3S-0324-FY57 | Human | P10 and R12, and optionally one or more of E15, I28, P32, N45 and K54 | IgG2 | Inhib | KD = 1 nM | |
| V3S-0324-FY58 | Human | P10 and Q48, optionally one of more of K54 and N45 | IgG2 | Inhib | KD = 3.7 nM |
| Description | This product is a human monoclonal antibody that reacts with CXCL12. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human, Mouse |
| Immunogen | Human chemokine (C-X-C motif) ligand 12 |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | WB; ELISA; Neut; FC |
| Application Notes | The antibody is recommended for detection of CXCL12 by WB, ELISA, Neut, FC assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | CXCL12 |
| Alternative Name | CXCL12; chemokine (C-X-C motif) ligand 12; IRH; PBSF; SDF1; TLSF; TPAR1; SCYB12; stromal cell-derived factor 1; intercrine reduced in hepatomas; pre-B cell growth-stimulating factor; anti-SDF-1 |
| Gene ID | 6387 |
| UniProt | P48061 |
| Related pathway | Apoptotic Pathways in Synovial Fibroblasts; PAK Pathway |
| Research Area | Immunology; Cardiovascular |
