| Description | This product is a monoclonal antibody derived from Human (Homo sapiens), which can specifically recognize C-X-C motif chemokine receptor 4. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Immunogen | Recombinant NIH3T3-CXCR4cells and/or peptides corresponding to CXCR4 extracellular N-term and loops |
| Epitope | within the N-terminus and loops of the extracellular domain (ECD) |
| Isotype | IgG1, κ |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | Block; Cyt; FC |
| Application Notes | The antibody is recommended for detection of CXCR4 by Block, Cyt, FC assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | CXCR4 |
| Alternative Name | Chemokine (C-X-C Motif) Receptor 4; Leukocyte-Derived Seven Transmembrane Domain Receptor; Lipopolysaccharide-Associated Protein 3; Stromal Cell-Derived Factor 1 Receptor; LPS-Associated Protein 3; SDF-1 Receptor; CD184 Antigen; Fusin; LAP-3; LESTR; NPYRL; FB22; HM89; LCR1; Seven-Transmembrane-Segment Receptor, Spleen; Chemokine (C-X-C Motif), Receptor 4 (Fusin); Seven Transmembrane Helix Receptor; Neuropeptide Y Receptor Y3; Neuropeptide Y3 Receptor; D2S201E; HSY3RR; NPYY3R; CXC-R4; CXCR-4; CD184; NPY3R; WHIMS; NPYR; LAP3; WHIM |
| Gene ID | 7852 |
| UniProt | P61073 |
| Research Area | Immunology; Microbiology |
| Related Disease | Cancer |
