| Description | This is an antibody clone Cy.004 specific for CyRPA (cysteine rich protective antigen) of Plasmodium falciparum, derived from chicken. This antibody reduced parasite growth in vitro growth inhibition activity. It shows inhibition efficiency against Plasmodium falciparum. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Plasmodium falciparum |
| Immunogen | The chickens were immunized with recombinant CyRPA. |
| Epitope | Cy.004 binds predominantly to the second blade, as well as contacting a flexible loop between blades 1 and 2. |
| Affinity | 2.8 μM |
| Isotype | Chimeric (chicken/human) IgG1 |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein G purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; SPR; Inhib |
| Application Notes | ELISA: 10 μg/mL Surface plasmon resonance (SPR): Purified mAb was immobilized on a protein G chip through a 30 s injection of 20 nM mAb. Inhibition assay (Inhib): Cy.004 inhibited growth with EC50 values of 170 μg/mL. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | CyRPA |
| Alternative Name | Cysteine rich protective antigen |
| Research Area | Infectious Disease |
| Related Disease | Malaria |
