Recombinant Anti-DDR1 Antibody (V3S-0522-YC5759) (CAT#: V3S-0522-YC5759)

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  • fig1
    Figure 1 Recombinant Anti-DDR1 Antibody (V3S-0522-YC5759) in SDS-PAGE.
  • fig1
    Figure 2 Recombinant Anti-DDR1 Antibody (V3S-0522-YC5759) in SEC-HPLC.
  • fig1
    Figure 3 Recombinant Anti-DDR1 Antibody (V3S-0522-YC5759) in ELISA.
  • fig1
    Figure 4 Recombinant Anti-DDR1 Antibody (V3S-0522-YC5759) in Western Blot.

Datasheet

MSDS

COA

Summary
Property
Applications
Protocols
Target

Summary

Description This product is a monoclonal antibody derived from Human (Homo sapiens), which can specifically recognize Discoidin domain receptor tyrosine kinase 1. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation.
Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality Monoclonal
Host Species Human
Target Species Human
Isotype IgG

Property

Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.

Applications

Application IF
Application Notes The antibody is recommended for detection of DDR1 by IF assay.

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target DDR1
Alternative Name DDR1; discoidin domain receptor tyrosine kinase 1; CAK; DDR; NEP; HGK2; PTK3; RTK6; TRKE; CD167; EDDR1; MCK10; NTRK4; PTK3A; epithelial discoidin domain-containing receptor 1; tyrosine kinase DDR; cell adhesion kinase; mammary carcinoma kinase 10; tyrosine-protein kinase CAK; protein-tyrosine kinase RTK-6; neuroepithelial tyrosine kinase; PTK3A protein tyrosine kinase 3A; CD167 antigen-like family member A; neurotrophic tyrosine kinase, receptor, type 4
Gene ID 780
UniProt Q08345
Research Area Neuroscience; Cancer Research
Related Disease Cancer

Tested Data

SDS-PAGE

Figure 1 Recombinant Anti-DDR1 Antibody (V3S-0522-YC5759) in SDS-PAGE.

Lane 1: Non-Reduced Antibody
Lane 2: Reduced Antibody

Figure 1 Recombinant Anti-DDR1 Antibody (V3S-0522-YC5759) in SDS-PAGE.

SEC-HPLC

Figure 2 Recombinant Anti-DDR1 Antibody (V3S-0522-YC5759) in SEC-HPLC.

The purity of V3S-0522-YC5759 was 99.0621% as determined by SEC-HPLC.

Figure 2 Recombinant Anti-DDR1 Antibody (V3S-0522-YC5759) in SEC-HPLC.

ELISA

Figure 3 Recombinant Anti-DDR1 Antibody (V3S-0522-YC5759) in ELISA.

ELISA analysis of V3S-0522-YC5759 was performed by coating with human DRR1 protein.

The secondary antibody: HRP-conjugated goat anti-human IgG

Figure 3 Recombinant Anti-DDR1 Antibody (V3S-0522-YC5759) in ELISA.

WB

Figure 4 Recombinant Anti-DDR1 Antibody (V3S-0522-YC5759) in Western Blot.

Western blot analysis of V3S-0522-YC5759 was performed by loading human DDR1 protein onto a 12% Tris-HCl polyacrylamide gel.

V3S-0522-YC5759 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-conjugated goat anti-human IgG
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)

Figure 4 Recombinant Anti-DDR1 Antibody (V3S-0522-YC5759) in Western Blot.

For research use only, not directly for clinical use.
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