| Description | This product is a human neutralizing antibody binding EBOV GP, named EBOV-293. It exhibits high affinity with EBOV glycoprotein and neutralization efficiency against Ebola virus. It binds glycan cap epitope on the EBOV glycoprotein. This antibody has been detected by Enzyme-linked immunosorbent assay, Flow cytometry and Neutralization assay. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | EBOV |
| Cross Reactivity | BDBV, SUDV |
| Epitope | Glycan cap epitope on the ebolavirus glycoprotein (GP) |
| IC50 | EBOV: 1.672 μg/mL BDBV: 1.892 μg/mL SUDV: 10.456 μg/mL |
| EC50 | EBOV: 49 ng/mL BDBV: 198 ng/mL SUDV: 91 ng/mL The EC50 value of GP binding was determined by ELISA assay. |
| Isotype | Human IgG1 |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; FC; Neut |
| Application Notes | For ELISA, purified mAb was diluted serially in blocking buffer, added to the wells and incubated for 1 h at ambient temperature. For Flow cytometry, Cells were washed with ice-cold FACS buffer, counted, seeded at ~5,000 to 20,000 viable cells per well in a V-bottom 96-well plate for each mAb to be tested and incubated 2 h at 4°C with 5 μg/mL of mAb in triplicate in a total volume 50 μL per staining. Neutralization assay: 0.2 mg/mL |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | EBOV GP |
| Alternative Name | Ebolavirus glycoprotein |
| Research Area | Infectious Disease |
| Related Disease | Severe hemorrhagic fever |
