| Description | This product is a neutralizing antibody clone CA45, derived from monkey. It binds with the ectodomain of Ebola virus (EBOV) GP (glycoprotein), that spans multiple faces of the fusion loop stem, across both GP1 and GP2 subunits. CA45 neutralizes EBOV, BDBV and SUDV with high potency and it protects against EBOV, SUDV, and BDBV virus challenge in mice, guinea pigs, or ferrets. This antibody has been tested by Enzyme-linked immunosorbent assay, Bio-layer interferometry and Neutralization assay. |
| Clonality | Monoclonal |
| Host Species | Monkey |
| Target Species | EBOV |
| Cross Reactivity | SUDV, BDBV, RESTV |
| Immunogen | The cynomolgus macaques immunized with trivalent cocktails of recombinant EBOV, SUDV, and Marburg virus (MARV) GPs. |
| Epitope | The CA45 epitope on GP was predominantly located in and around the fusion loop stem, made up of strand β19-β20 positioned along the equatorial GP1-GP2 interface of GP. |
| IC50 | BDBV: 0.9-2.8 nM EBOV: 3-4.4 nM SUDV: 3.3-12 nM RESTV: 42 nM |
| Affinity | EBOV: 10.6 nM SUDV: 3.3 nM BDBV: 1.2 nM RESTV: 161 nM |
| Isotype | Cynomolgus IgG |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; BLI; Neut |
| Application Notes | ELISA: 0.4 μg/mL |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | EBOV GP |
| Alternative Name | Ebolavirus glycoprotein |
| Research Area | Infectious Disease |
| Related Disease | Severe hemorrhagic fever |
