| Description | This product is a monoclonal antibody derived from Mouse (Mus musculus), which can specifically recognize Envelope glycoprotein H and Envelope glycoprotein L. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Epstein-Barr Virus (EBV) |
| Epitope | The epitope is gH D-II. |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; FC; Neut |
| Application Notes | The antibody is recommended for detection of EBV gH / gL by ELISA, FC, Neut assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | EBV gH / gL |
| Alternative Name | EBV gH/gL; Envelope glycoprotein H; Envelope glycoprotein L |
| Research Area | Infectious Disease |
Figure 1 Anti-EBV gH / gL Neutralizing Antibody (V3S-0522-YC890) in SDS-PAGE.
SDS-PAGE analysis of V3S-0522-YC890 in β-mercaptoethanol-reduced (Lane 1, 1.5 μg) and non-reduced (Lane 2, 1.5 μg) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
Figure 1 Anti-EBV gH / gL Neutralizing Antibody (V3S-0522-YC890) in SDS-PAGE.
Figure 2 Anti-EBV gH / gL Neutralizing Antibody (V3S-0522-YC890) in Western Blot.
WB analysis of V3S-0522-YC890 was performed by coating with Epstein-Barr virus (EBV) (strain B95-8) gH & gL Heterodimer protein (His & Flag Tag).
Figure 2 Anti-EBV gH / gL Neutralizing Antibody (V3S-0522-YC890) in Western Blot.
Figure 3 Anti-EBV gH / gL Neutralizing Antibody (V3S-0522-YC890) in ELISA.
ELISA analysis of V3S-0522-YC890 was performed by coating with Epstein-Barr virus (EBV) (strain B95-8) gH & gL Heterodimer protein (His & Flag Tag).
Figure 3 Anti-EBV gH / gL Neutralizing Antibody (V3S-0522-YC890) in ELISA.
Figure 4 Anti-EBV gH / gL Neutralizing Antibody (V3S-0522-YC890) in Western Blot.
Western blot analysis of V3S-0522-YC890 was performed with Epstein-Barr virus Glycoprotein H & Glycoprotein L Heterodimer protein, Twin-Strep&His Tag.
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)
The secondary antibody: HRP goat anti-mouse IgG (H+L)
Figure 4 Anti-EBV gH / gL Neutralizing Antibody (V3S-0522-YC890) in Western Blot.
Figure 5 Anti-EBV gH / gL Neutralizing Antibody (V3S-0522-YC890) in Dot Blot.
Dot blot analysis of V3S-0522-YC890 was performed with Epstein-Barr virus Glycoprotein H & Glycoprotein L Heterodimer protein, Twin-Strep&His Tag.
The secondary antibody: HRP goat anti-mouse IgG (H+L)
Figure 5 Anti-EBV gH / gL Neutralizing Antibody (V3S-0522-YC890) in Dot Blot.
