| Description | This product is a recombinant Human antibody provided by CreativeBiolabs. The antibody is specific for EPH Receptor A2. It can be used for EPH Receptor A2 detection in Enzyme-Linked Immunosorbent Assay, Flow Cytometry and Immunofluorescence. It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultrapurified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Affinity | The affinity is in nanomolar range, about 180 nM, determined by surface plasmon resonance. |
| Isotype | IgG |
| Expression Species | HEK293F or CHO Cell line |
| Conjugation | Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form. |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Purified with Protein A or G affinity chromatography |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; FC; IF |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | EPHA2 |
| Alternative Name | EPH Receptor A2; Tyrosine-Protein Kinase Receptor ECK; EC 2.7.10.1; ECK; Epithelial Cell Receptor Protein Tyrosine Kinase; Soluble EPHA2 Variant 1; Epithelial Cell Kinase; EC 2.7.10; CTRCT6; ARCC2; CTPP1; EphA2; CTPA |
| Gene ID | 1969 |
| UniProt | P29317 |
| Research Area | Cardiovascular; Neuroscience |
| Related Disease | Cancer |
Figure 1 Recombinant Anti-EPHA2 Antibody (V3S-1022-YC3129) in Western Blot.
Western blot analysis of V3S-1022-YC3129 was performed by loading EPHA2 protein (His tag) onto a 12% Tris-HCl polyacrylamide gel. proteins were transferred to a NC membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-1022-YC3129 and HRP conjugated anti-human IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)
Figure 1 Recombinant Anti-EPHA2 Antibody (V3S-1022-YC3129) in Western Blot.
Figure 2 Recombinant Anti-EPHA2 Antibody (V3S-1022-YC3129) in Dot Blot.
Dot blot analysis of V3S-1022-YC3129 was performed by coating with EPHA2 protein (His tag).
V3S-1022-YC3129 incubation concentration: 2 μg/mL.
HRP conjugated anti-human IgG as a secondary antibody (1: 2000)
Figure 2 Recombinant Anti-EPHA2 Antibody (V3S-1022-YC3129) in Dot Blot.
Figure 3 Recombinant Anti-EPHA2 Antibody (V3S-1022-YC3129) in ELISA.
ELISA analysis of V3S-1022-YC3129 was performed by coating with EPHA2 protein (His tag). Then blocked with BSA and incubated with V3S-1022-YC3129. The HRP conjugated anti-human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 3 Recombinant Anti-EPHA2 Antibody (V3S-1022-YC3129) in ELISA.
