| Description | This product is a monoclonal antibody derived from Mouse (Mus musculus), which can specifically recognize EPH receptor B3. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Immunogen | Recombinant form of the extracellular domain (ECD) (corresponding to amino acids 37-558) of human EphB3 |
| Epitope | Within the ECD domain. |
| Affinity | KD = 0.6 nM |
| Isotype | IgG, κ |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | WB; IP; FC |
| Application Notes | The antibody is recommended for detection of EPHB3 by WB, IP, FC assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | EPHB3 |
| Alternative Name | EPH Receptor B3; Tyrosine-Protein Kinase TYRO6; EPH-Like Tyrosine Kinase 2; Embryonic Kinase 2; EPH-Like Kinase 2; EC 2.7.10.1; TYRO6; ETK2; HEK2; EK2; EPH-Like Tyrosine Kinase-2; Human Embryo Kinase 2; EC 2.7.10; EphB3 |
| Gene ID | 2049 |
| UniProt | P54753 |
| Research Area | Cardiovascular |
| Related Disease | Lung, ovarian, esophageal, colon and breast cancer |
