Description | This product is a humanized monoclonal antibody that reacts with F glycoprotein of Hendra or Nipah virus. The humanized h5B3.1 maintained all binding activities with F glycoprotein as well as virus neutralizing titer as compared to its parental antibody. It recognizes a conformation-dependent epitope and can be used to distinguish pre and post fusion F. |
Clonality | Monoclonal |
Host Species | Human |
Target Species | Hendra henipavirus, Nipah henipavirus |
Affinity | 62 nM |
Isotype | IgG1 kappa |
Expression Species | HEK293F or CHO |
Conjugation | None |
Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
Endotoxin | <1 EU/mg |
Purification | Protein A affinity purified |
Sterility | 0.2 μM filtered |
Formulation | PBS, pH 7.4 |
Preservation | No preservatives |
Stabilizer | No stabilizers |
Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
Application | Inhib; WB; IP |
Application Notes | The antibody is recommended for detection of F Protein by Inhib, WB (1/1000-1/5000), IP assays. |
ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
WB | Western Blot Protocol |
FC | Flow Cytometry Protocol |