| Description | This product is a monoclonal antibody derived from rabbit, which can specifically recognize FASLG. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Rabbit |
| Target Species | Human |
| Immunogen | A synthetic N-terminal peptide, encoding the first 21aa of human CD95L (CD95L_NT 1-21: MQQPFNYPYPQIYWVDSSASS) |
| Epitope | in the N-terminus (aa 1-21) |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; IHC |
| Application Notes | The antibody is recommended for detection of FASLG by ELISA, IHC assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | FASLG |
| Alternative Name | FASLG; Fas ligand (TNF superfamily, member 6); APTL; FASL; CD178; CD95L; ALPS1B; CD95-L; TNFSF6; APT1LG1; tumor necrosis factor ligand superfamily member 6; CD95 ligand; fas antigen ligand; apoptosis antigen ligand; apoptosis (APO-1) antigen ligand 1 |
| Gene ID | 356 |
| UniProt | P48023 |
| Related pathway | FAS/FASLG signaling pathway |
| Research Area | Cell Biology |
