| Description | This product is a human monoclonal antibody that reacts with FCER2. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | WB; Neut; ELISA; IF; IP; FuncS; FC |
| Application Notes | The antibody is recommended for detection of FCER2 by WB, Neut, ELISA, IF, IP, FuncS, FC assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | FCER2 |
| Alternative Name | FCER2; Fc fragment of IgE, low affinity II, receptor for (CD23); CD23A, Fc fragment of IgE, low affinity II, receptor for (CD23A), FCE2; low affinity immunoglobulin epsilon Fc receptor; CD23; CLEC4J; BLAST-2; CD23 antigen; fc-epsilon-RII; |
| Gene ID | 2208 |
| UniProt | P06734 |
| Research Area | Cell Biology; Immunology |
Figure 1 Anti-FCER2 Monoclonal Antibody (V3S-0622-YC4458) in ELISA
ELISA analysis of V3S-0622-YC4458 was performed by coating with Recombinant Human CD23 Protein. Then blocked with BSA, and incubated with Anti-Human CD23 Antibody (V3S-0622-YC4458). The HRP-conjugated anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 1 Anti-FCER2 Monoclonal Antibody (V3S-0622-YC4458) in ELISA
Figure 2 Anti-FCER2 Monoclonal Antibody (V3S-0622-YC4458) in WB
Western blot analysis of V3S-0622-YC4458 was performed by loading Recombinant Human CD23 Protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0622-YC4458 and HRP Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.
Lane 1: Reducing antigen (0.1 μg)
Lane 2: Reducing antigen (0.3 μg)
Lane 3: Reducing antigen (0.6 μg)
Figure 2 Anti-FCER2 Monoclonal Antibody (V3S-0622-YC4458) in WB
