Recombinant Anti-FCGR2A (Ig-like domain 2) Antibody (V3S-0522-YC6593) (CAT#: V3S-0522-YC6593)

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Description This product is a monoclonal antibody derived from Human (Homo sapiens), which can specifically recognize Fc fragment of IgG receptor Iia. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation.
Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality Monoclonal
Host Species Human
Target Species Human
Immunogen A fusion protein comprised of FcɣRIIa-H131 (FcɣRIIa with an arginine (R) to histidine (H) substitution residue 131) conjugated to human serum albumin
Epitope Located in the second Ig-like domain of FcɣRIIa that is critically dependent on the presence of a histidine at position 131.
Isotype IgG, κ


Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.


Application ELISA; FC; IHC
Application Notes The antibody is recommended for detection of FCGR2A by ELISA, FC, IHC assays.


ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol


Target FCGR2A
Alternative Name Fc Fragment Of IgG Receptor IIa; Fc Fragment Of IgG, Low Affinity IIa, Receptor (CD32); Immunoglobulin G Fc Receptor II; Fc Gamma Receptor IIa; IgG Fc Receptor II-A; Fc-Gamma RII-A; Fc-Gamma-RIIa; FcRII-A; FCGR2A1; CDw32; IGFR2; FCG2; CD32; Fc Fragment Of IgG, Low Affinity IIa, Receptor For (CD32); CD32 Antigen; CD32A; FCGR2; FcGR
Gene ID 2212
UniProt P12318
Research Area Immunology; Stem Cell
Related Disease Auto-immune, Infectious disease
For research use only, not directly for clinical use.
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