| Description | This product is a monoclonal antibody derived from Human (Homo sapiens), which can specifically recognize Fc fragment of IgG receptor Iia. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Immunogen | A fusion protein comprised of FcɣRIIa-H131 (FcɣRIIa with an arginine (R) to histidine (H) substitution residue 131) conjugated to human serum albumin |
| Epitope | Located in the second Ig-like domain of FcɣRIIa that is critically dependent on the presence of a histidine at position 131. |
| Isotype | IgG, κ |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; FC; IHC |
| Application Notes | The antibody is recommended for detection of FCGR2A by ELISA, FC, IHC assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | FCGR2A |
| Alternative Name | Fc Fragment Of IgG Receptor IIa; Fc Fragment Of IgG, Low Affinity IIa, Receptor (CD32); Immunoglobulin G Fc Receptor II; Fc Gamma Receptor IIa; IgG Fc Receptor II-A; Fc-Gamma RII-A; Fc-Gamma-RIIa; FcRII-A; FCGR2A1; CDw32; IGFR2; FCG2; CD32; Fc Fragment Of IgG, Low Affinity IIa, Receptor For (CD32); CD32 Antigen; CD32A; FCGR2; FcGR |
| Gene ID | 2212 |
| UniProt | P12318 |
| Research Area | Immunology; Stem Cell |
| Related Disease | Auto-immune, Infectious disease |
