Anti-FLT3 (domain 5) Neutralizing Antibody (V3S-0522-YC556) (CAT#: V3S-0522-YC556)

Figure 1 Anti-FLT3 Monoclonal Antibody (V3S-0522-YC556) in SDS-PAGE

SDS-PAGE analysis of V3S-0522-YC556 in non-reduced (lane 1) and β-mercaptoethanol-reduced (lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as about 180 kDa.

Figure 2 Anti-FLT3 Monoclonal Antibody (V3S-0522-YC556) in WB

Western blot analysis of V3S-0522-YC556 was performed by loading 2 µg onto a 12% Tris-HCl polyacrylamide gel in non-reduced (lane 1) and β-mercaptoethanol-reduced (lane 2) conditions. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with Goat Anti-Human IgG HRP secondary antibody at a dilution of 1:8,000 for 75 minutes. Chemiluminescent detection was performed.

Figure 3 Anti-FLT3 Monoclonal Antibody (V3S-0522-YC556) in SEC-HPLC

The purity of V3S-0522-YC556 was greater than 90% as determined by SEC-HPLC.

Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.3
Detection: UV 280 nm
Injection: 25 µl

Figure 4 Anti-FLT3 Monoclonal Antibody (V3S-0522-YC556) in WB

Western blot analysis was performed by loading 1 µg (lane 1) and 1 µg (lane 2) recombinant human FLT3 antigen onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least 1 hour. Membranes were probed with TLT3 antibody (V3S-0522-YC556) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Then probed with Goat anti-Human IgG HRP secondary antibody at a dilution of 1:8,000 for one hour. Chemiluminescent was detected.

Figure 5 Anti-FLT3 Monoclonal Antibody (V3S-0522-YC556) in ELISA

ELISA analysis of V3S-0522-YC556 was performed by coating with recombinant human FLT3 antigen. Then blocked with BSA, and incubated with TLT3 antibody (V3S-0522-YC556) at a starting concentration of 1µg/mL. The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.