Description | This product is a human monoclonal antibody that reacts with FXII. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
Clonality | Monoclonal |
Host Species | Human |
Target Species | Human |
Affinity | 1.56 nM |
Isotype | IgG4 lambda |
Expression Species | HEK293F or CHO |
Conjugation | None |
Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
Endotoxin | <1 EU/mg, determined by LAL method |
Purification | Protein A affinity purified |
Sterility | 0.2 μM filtered |
Formulation | PBS, pH 7.4 |
Preservation | No preservatives |
Stabilizer | No stabilizers |
Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
Application | WB; ELISA |
Application Notes | The antibody is recommended for detection of FXII by ELISA assay. |
ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
WB | Western Blot Protocol |
FC | Flow Cytometry Protocol |
Target | FXII |
Alternative Name | FXII; factor XII; Hageman factor; plasma protein factor XII; plasma protein FXII |
Figure 1 Anti-FXII Monoclonal Antibody (V3S-0622-YC3322) in SDS-PAGE
SDS-PAGE analysis of V3S-0622-YC3322 in non-reduced and β-mercaptoethanol-reduced conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
Figure 1 Anti-FXII Monoclonal Antibody (V3S-0622-YC3322) in SDS-PAGE
Figure 2 Anti-FXII Monoclonal Antibody (V3S-0622-YC3322) in WB
Western blot analysis of V3S-0622-YC3322 was performed by loading 0.2 µg onto a 12% Tris-HCl polyacrylamide gel in non-reduced (lane 1) and β-mercaptoethanol-reduced (lane 2) conditions. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with Goat Anti-Human IgG HRP secondary antibody at a dilution of 1:8,000 for 75 minutes. Chemiluminescent detection was performed.
Figure 2 Anti-FXII Monoclonal Antibody (V3S-0622-YC3322) in WB
Figure 3 Anti-FXII Monoclonal Antibody (V3S-0622-YC3322) in WB
Western blot analysis of V3S-0622-YC3322 was performed by loading Recombinant Human FXII Protein (lane 1: 0.1 μg, lane 2: 0.5 μg, lane 3: 1.0 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0622-YC3322 and HRP Goat Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.
Figure 3 Anti-FXII Monoclonal Antibody (V3S-0622-YC3322) in WB
Figure 4 Anti-FXII Monoclonal Antibody (V3S-0622-YC3322) in ELISA
ELISA analysis of V3S-0622-YC3322 was performed by coating with Recombinant Human FXII Protein. Then blocked with BSA, and incubated with Anti-FXII Antibody (V3S-0622-YC3322). The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 4 Anti-FXII Monoclonal Antibody (V3S-0622-YC3322) in ELISA