Description | This product is a recombinant Human antibody provided by CreativeBiolabs. The antibody is specific for Human Cytomegalovirus UL128. It can be used for Human Cytomegalovirus UL128 detection in Enzyme-Linked Immunosorbent Assay and Neutralization Assay. It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultrapurified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. |
Clonality | Monoclonal |
Host Species | Human |
Target Species | Human Cytomegalovirus (HCMV) |
Isotype | IgG |
Expression Species | HEK293F or CHO Cell line |
Conjugation | Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form. |
Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
Endotoxin | <1 EU/mg, determined by LAL method |
Purification | Purified with Protein A or G affinity chromatography |
Sterility | 0.2 μM filtered |
Formulation | PBS, pH 7.4 |
Preservation | No preservatives |
Stabilizer | No stabilizers |
Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
Application | WB; DB; ELISA; Neut |
ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
WB | Western Blot Protocol |
FC | Flow Cytometry Protocol |
Target | HCMV UL128 |
Alternative Name | hCMV |
Research Area | Cell Biology |
Related Disease | Chronic diseases |
Figure 1 Anti-HCMV UL128 Monoclonal Antibody (V3S-1022-YC1920) in WB
Western blot analysis of V3S-1022-YC1920 was performed by loading Recombinant Human cytomegalovirus Uncharacterized protein UL128 (His tag, 23.7 kDa) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-1022-YC1920 and HRP goat anti-Human IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)
Figure 1 Anti-HCMV UL128 Monoclonal Antibody (V3S-1022-YC1920) in WB
Figure 2 Anti-HCMV UL128 Monoclonal Antibody (V3S-1022-YC1920) in DB
Dot Blot analysis of V3S-1022-YC1920 was performed by coating with Recombinant Human cytomegalovirus Uncharacterized protein UL128 (His tag, 23.7 kDa).
V3S-1022-YC1920 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Human IgG as a secondary antibody (1: 2000)
Figure 2 Anti-HCMV UL128 Monoclonal Antibody (V3S-1022-YC1920) in DB
Figure 3 Anti-HCMV UL128 Monoclonal Antibody (V3S-1022-YC1920) in ELISA
ELISA analysis of V3S-1022-YC1920 was performed by coating with Recombinant Human cytomegalovirus Uncharacterized protein UL128 (His tag, 23.7 kDa). Then blocked with BSA and incubated with V3S-1022-YC1920. The HRP-conjugated goat anti-Human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 3 Anti-HCMV UL128 Monoclonal Antibody (V3S-1022-YC1920) in ELISA