| Description | This is a anti-HIV-1 human antibody recoginzing membrane-proximal external region (MPER) of HIV-1 gp41. It is an IgG1 that has a relatively short CDRH3 with limited hydrophobicity. It has broad neutralizing ability against HIV-1. The IC₅₀ value of this antibody against HIV-1 is 5.8 μg/mL. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | HIV-1 |
| Epitope | The membrane-proximal external region (MPER) of HIV-1 Env (aa 671-683). L663A, D664A, S665A, W666A, K667A, L669A, W670A, K677A, and W678A mutations of MPER enhanced HIV neutralization by the bnAb. |
| IC50 | 5.8 μg/mL |
| Affinity | KD = 9.9 nM |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO cell |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; BLI; FC; Neut |
| Application Notes | Neutralization assay (Neut): The IC₅₀ value of this antibody against HIV-1 is 5.8 μg/mL. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | HIV-1 Env MPER |
| Alternative Name | Human immunodeficiency virus type 1 envelope; HIV-1; Env |
| Research Area | Infectious Disease |
| Related Disease | Acquired Immune Deficiency Syndrome |
Figure 1 Binding ability of PGZL1 antibody to MPER peptide measured by BLI.
PGZL1 antibody has potent affinity against MPER peptide.
Figure 1 Binding ability of PGZL1 antibody to MPER peptide measured by BLI.
Figure 2 The effect of PGZL1 antibody to neutralize Du156.12 and HxB2.
The antibody has potent neutralizing ability against HIV.
Figure 2 The effect of PGZL1 antibody to neutralize Du156.12 and HxB2.
