| Description | This antibody is a mouse neutralizing antibody clone F-6 binding with HIV-1 RT. It binds to the carboxyl terminus of the large subunit of RT, which contains the ribonuclease H (RNase H) domain, and not the polymerase domain of the protein. F-6 inhibits the RNA-dependent DNA polymerase (RDDP) activity of RT and inhibits also its DNA-dependent DNA polymerase (DDDP) activity. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | HIV-1 |
| Epitope | F-6 binds to the carboxyl terminus of the large subunit of RT, which contains the ribonuclease H (RNase H) domain, and not the polymerase domain of the protein. |
| Neutralization Breath | Inhibited 55% of the HIV-1 RT activity. |
| IC50 | 60 nM (Inhibition of RDDP activity) |
| Affinity | 43.87 nM |
| Isotype | Human IgG |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; WB; SPR |
| Application Notes | Western blot (WB): The nitrocellulose was washed with PBS and incubated with the antibody overnight at 4°C. Surface plasmon resonance (SPR): Immobilization resulted in 5600 response units and various concentrations of antibody in PBS were injected over the surface at 30 μL/min at 25°C. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | HIV-1 RT p66 |
| Alternative Name | Human immunodeficiency virus type 1 reverse transcriptase |
| Research Area | Infectious Disease |
| Related Disease | Acquired Immune Deficiency Syndrome |
