| Description | This product is a monoclonal antibody derived from Rhesus, which can specifically recognize HIV1 gp120. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Rhesus |
| Target Species | Human Immunodeficiency Virus 1 (HIV1) |
| Immunogen | Soluble gp140-F trimers |
| Affinity | 8 nM for gp120, 1.9 nM for gp120 core, 6.4 nM for TriMut |
| Isotype | IgG kappa |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; Neut |
| Application Notes | The antibody is recommended for detection of HIV1 gp120 by ELISA, Neut assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | HIV1 gp120 |
| Alternative Name | HIV-1 envelope glycoprotein gp120; gp120; HIV-1 gp120; HIV-1 |
| Research Area | Infectious Disease |
