| Description | This product is a human monoclonal antibody that reacts with HMGB1. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Cow |
| Immunogen | A mixture of immunization commercial bovine thymus-derived HMGB1 and HMGB2 rats |
| Epitope | Located in EEEDDDDE of HMGB1. |
| Affinity | 0.329 nM |
| Isotype | IgG1 lambda |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA |
| Application Notes | The antibody is recommended for detection of HMGB1 by ELISA assay. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | HMGB1 |
| Alternative Name | HMGB1; high mobility group box 1; HMG1; HMG3; SBP-1; high mobility group protein B1; HMG-1; Amphoterin |
| Gene ID | 3146 |
| UniProt | A0A024RDR0 |
| Research Area | Epigenetics |
