| Description | This antibody is a neutralizing IgG clone MPV467 specific for hMPV (Human metapneumovirus) F protein, derived from human. MPV467 has potent neutralizing activity against Human metapneumovirus. mAbs MPV467 has the most potent neutralizing activity against both hMPV CAN/97-83 and hMPV TN/93-32, with picomolar activity (below 1 ng/mL) against hMPV TN/93-32. It is one of the most potent mAb, which limits virus replication in mouse lungs in mice assay. This antibody has been tested using Enzyme-linked immunosorbent assay and Neutralization assay. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | hMPV |
| Cross Reactivity | hMPV CAN/97-83, hMPV TN/93-32 |
| Epitope | It binds an epitope located across antigenic sites II and V. |
| IC50 | 5 ng/mL (hMPV CAN/97-83) 0.6 ng/mL (hMPV TN/93-32) |
| EC50 | 380 ng/mL (hMPV A1 F) 120 ng/mL (hMPV A2 F) 120 ng/mL (hMPV B1 F) 370 ng/mL (hMPV B2 F) 53 ng/mL (hMPV A1 F monomer) 260 ng/mL (hMPV A1 F trimer (heated)) 60 ng/mL (hMPV B2 F monomer) 650 ng/mL (hMPV B2 F trimer (heated)) 61 ng/mL (hMPV B2 F GCN4) |
| Isotype | Human IgG |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; Neut |
| Application Notes | ELISA: A total of 25 μL of the serially diluted antibody was added to the wells and incubated for 1 h at room temperature. Neutralization assay (Neut): Serially diluted sterile-filtered mAb was added to the suspension of either of hMPV strains, CAN/97-83 and TN/93-32, in equal amounts (1:1) and incubated for 1 h on the day of the experiment. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | hMPV F Protein |
| Alternative Name | Human metapneumovirus fusion protein |
| Research Area | Infectious Disease |
| Related Disease | Respiratory disease |
