Description | This product is a human monoclonal antibody that reacts with HSP90B1. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
Clonality | Monoclonal |
Host Species | Human |
Target Species | Human |
Isotype | IgG1 kappa |
Expression Species | HEK293F or CHO |
Conjugation | None |
Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
Endotoxin | <1 EU/mg, determined by LAL method |
Purification | Protein A affinity purified |
Sterility | 0.2 μM filtered |
Formulation | PBS, pH 7.4 |
Preservation | No preservatives |
Stabilizer | No stabilizers |
Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
Application | WB |
Application Notes | The antibody is recommended for detection of HSP90B1 by WB assay. |
ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
WB | Western Blot Protocol |
FC | Flow Cytometry Protocol |
Target | HSP90B1 |
Alternative Name | HSP90B1; heat shock protein 90kDa beta (Grp94); member 1; ECGP; GP96; TRA1; GRP94; HEL35; HEL-S-125m; endoplasmin; tumor rejection antigen 1; epididymis luminal protein 35; 94 kDa glucose-regulated protein; tumor rejection antigen (gp96) 1; endothelial cell (HBMEC) glycoprotein; heat shock protein 90 kDa beta member 1; stress-inducible tumor rejection antigen gp96; epididymis secretory sperm binding protein Li 125m; anti-GRP94 |
Gene ID | 7184 |
UniProt | P14625 |
Research Area | Signal Pathway |