| Description | This product is a monoclonal antibody derived from Mouse (Mus musculus), which can specifically recognize Influenza A virus H3 subtype hemagglutinin. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Influenza A virus (IAV) |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | DB; ELISA |
| Application Notes | The antibody is recommended for detection of IAV H3 Subtype HA by ELISA assay. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | IAV H3 Subtype HA |
| Alternative Name | Influenza A Virus Hemagglutinin H3 HA |
| Research Area | Microbiology; Infectious Disease |
| Related Disease | Influenza virus infection |
Figure 1 Anti-Influenza A Virus Hemagglutinin H3 HA Monoclonal Antibody (V3S-0522-YC3520) in SDS-PAGE
SDS-PAGE analysis of V3S-0522-YC3520 in non-reduced (Lane 1) and β-mercaptoethanol-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 180-245 kDa.
Figure 1 Anti-Influenza A Virus Hemagglutinin H3 HA Monoclonal Antibody (V3S-0522-YC3520) in SDS-PAGE
Figure 2 Anti-Influenza A Virus Hemagglutinin H3 HA Monoclonal Antibody (V3S-0522-YC3520) in SEC-HPLC
SEC-HPLC analysis of V3S-0522-YC3520 shows that the purity is more than 95%.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
Figure 2 Anti-Influenza A Virus Hemagglutinin H3 HA Monoclonal Antibody (V3S-0522-YC3520) in SEC-HPLC
Figure 3 Anti-Influenza A Virus Hemagglutinin H3 HA Monoclonal Antibody (V3S-0522-YC3520) in ELISA
ELISA analysis of V3S-0522-YC3520 was performed by coating with Influenza A H3N2 (A/Perth/16/2009) Hemagglutinin / HA Protein (His Tag). Then blocked with BSA and incubated with V3S-0522-YC3520. The HRP-conjugated goat anti-Mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 3 Anti-Influenza A Virus Hemagglutinin H3 HA Monoclonal Antibody (V3S-0522-YC3520) in ELISA
Figure 4 Anti-Influenza A Virus Hemagglutinin H3 HA Monoclonal Antibody (V3S-0522-YC3520) in ELISA
ELISA analysis of V3S-0522-YC3520 was performed by coating with Influenza A H3N2 (A/Victoria/210/2009) Hemagglutinin / HA Protein (His Tag). Then blocked with BSA and incubated with V3S-0522-YC3520. The HRP-conjugated goat anti-Mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 4 Anti-Influenza A Virus Hemagglutinin H3 HA Monoclonal Antibody (V3S-0522-YC3520) in ELISA
Figure 5 Anti-Influenza A Virus Hemagglutinin H3 HA Monoclonal Antibody (V3S-0522-YC3520) in DB
Dot Blot analysis of V3S-0522-YC3520 was performed by coating with Influenza A H3N2 (A/Perth/16/2009) Hemagglutinin / HA Protein (His Tag).
V3S-0522-YC3520 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Mouse IgG as a secondary antibody (1: 2000)
Figure 5 Anti-Influenza A Virus Hemagglutinin H3 HA Monoclonal Antibody (V3S-0522-YC3520) in DB
Figure 6 Anti-Influenza A Virus Hemagglutinin H3 HA Monoclonal Antibody (V3S-0522-YC3520) in DB
Dot Blot analysis of V3S-0522-YC3520 was performed by coating with Influenza A H3N2 (A/Victoria/210/2009) Hemagglutinin / HA Protein (His Tag).
V3S-0522-YC3520 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-Mouse IgG as a secondary antibody (1: 2000)
Figure 6 Anti-Influenza A Virus Hemagglutinin H3 HA Monoclonal Antibody (V3S-0522-YC3520) in DB
