| Description | This product is a human neutralizing antibody binding IAV NA, termed Z2B3. It exhibited cross-reactivity to both N9 and a broad range of seasonal and avian N1 proteins. It is capable of neutralizing multiple influenza virus. This antibody has been determined by Enzyme-linked immunosorbent assay, Surface plasmon resonance, Inhibition assay and Neutralization assay. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | IAV (N1, N9) |
| Cross Reactivity | 18N1, AH-N9, H1N1 (A/California/07/2009 and A/Puerto Rico/8/1934), H5N1 (A/Vietnam/1194/2004), H7N9 (A/Anhui/1/2013) |
| Epitope | The antibody Z2B3 recognizes seven of the eight conserved catalytic residues (except E276) and some of the frame residues (including W178, S179, I222, and E227) as its epitope in 18N1. The clone is sensitive to N1 NA with a K432E substitution. |
| IC50 | Inhibition of viral replication: 1.432 μg/ml (A/California/07/2009 (H1N1)) 5.202 μg/ml (A/Puerto Rico/8/1934 (H1N1)) 123.7 μg/ml (A/Serbia/NS-601/2014 (H1N1)) 7.883 μg/ml (A/Vietnam/1194/2004 (H5N1)) 27.27 μg/ml (A/Anhui/1/2013 (H7N9)) Inhibition of enzymatic activity: 30.41 nM (A/California/07/2009 (H1N1)) 33.07 nM (A/Puerto Rico/8/1934 (H1N1)) >2,000 nM (A/Serbia/NS-601/2014 (H1N1)) 69.73 nM (A/Vietnam/1194/2004 (H5N1)) 101.3 nM (A/Anhui/1/2013 (H7N9)) |
| Affinity | 543 nM |
| Isotype | Human IgG |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; SPR; Neut; Inhib |
| Application Notes | Surface plasmon resonance (SPR): Two-fold serial dilutions of antibody was prepared and sequentially injected at a flow rate of 30 μL/min for 60 s. Inhibition assay (Inhib): Two-fold serially diluted MAb was mixed with an equal volume of 100 TCID50 virus for 1 h at 37°C. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | IAV NA |
| Alternative Name | Neuraminidase |
| Research Area | Infectious Disease |
| Related Disease | Influenza |
