| Description | The product is a human antibody capable of binding to IgE Fc and was characterized in vitro using binding, signaling and functional assay systems. The clone MEDI4212 binds human IgE with an affinity of 1.95 pM. MEDI4212 inhibits IgE-mediated responses via FcϵRI and CD23. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Epitope | Amino acid residues Leu221, Arg223, Glu271 through to Asn275 inclusive, Ala309 to Thr315 inclusive, Thr317, Ser318 in Cɛ3 domain, and Glu353 in Cɛ4 domain. |
| IC50 | 8 nM |
| Affinity | KD = 1.95 pM |
| Isotype | Human IgG1 |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; WB; Neut; FuncS |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | IgE Fc |
| Alternative Name | IgE Fc; IgE; Immunoglobulin E; Immunoglobulin E Fc |
| Research Area | Immunology |
