| Description | This product is a human monoclonal antibody that reacts with IL12. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Immunogen | Human recombinant IL12 |
| Isotype | IgG1 kappa |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA |
| Application Notes | The antibody is recommended for detection of IL12 by ELISA assay. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | IL12 |
| Alternative Name | IL12; IL12A; IL12B; Interleukin 12 |
| Research Area | Immunology |
Figure 1 Recombinant Anti-IL12 Antibody (V3S-0622-YC3476) Fab Fragment in Western Blot.
Western blot analysis of IL12 Antibody (Fab) was performed with recombinant human IL2
protein.
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)
The secondary antibody: HRP goat anti-human IgG (H+L)
Figure 1 Recombinant Anti-IL12 Antibody (V3S-0622-YC3476) Fab Fragment in Western Blot.
Figure 2 Recombinant Anti-IL12 Antibody (V3S-0622-YC3476) Fab Fragment in ELISA.
ELISA analysis was performed by coating with recombinant human IL12 protein.
The secondary antibody: HRP goat anti-human IgG (H+L)
Figure 2 Recombinant Anti-IL12 Antibody (V3S-0622-YC3476) Fab Fragment in ELISA.
