| Description | This product is a human monoclonal antibody that reacts with IL1B. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Immunogen | Recombinant human ILI beta |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | WB; ELISA; IP; FC; FuncS; Neut; IF |
| Application Notes | The antibody is recommended for detection of IL1B by WB, ELISA, IP, FC, FuncS, Neut, IF assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | IL1B |
| Alternative Name | Hematopoietin 1; IFN beta inducing factor; IL 1; IL 1 beta; IL 1B; IL-1 beta; IL1 BETA; IL1F2; Interleukin 1 beta; Interleukin 1 beta precursor; Interleukin-1 beta; LAF; OAF; Osteoclast activating factor; OTTHUM |
| Gene ID | 3553 |
| Research Area | Cardiovascular |
Figure 1 Anti-IL1B Monoclonal Antibody (V3S-0622-YC1902) in SDS-PAGE
SDS-PAGE analysis of V3S-0622-YC1902 in non-reduced (lane 1) and β-mercaptoethanol-reduced (lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
Figure 1 Anti-IL1B Monoclonal Antibody (V3S-0622-YC1902) in SDS-PAGE
Figure 2 Anti-IL1B Monoclonal Antibody (V3S-0622-YC1902) in SEC-HPLC
The purity of V3S-0622-YC1902 was greater than 95% as determined by SEC-HPLC.
Figure 2 Anti-IL1B Monoclonal Antibody (V3S-0622-YC1902) in SEC-HPLC
Figure 3 Anti-IL1B Monoclonal Antibody (V3S-0622-YC1902) in WB
Western blot analysis of V3S-0622-YC1902 was performed by loading Recombinant Human IL1 Beta.
Lane 1: Non-Reducing Antigen (1 μg)
Lane 2: Reducing Antigen (1 μg)
Figure 3 Anti-IL1B Monoclonal Antibody (V3S-0622-YC1902) in WB
Figure 4 Anti-IL1B Monoclonal Antibody (V3S-0622-YC1902) in ELISA
ELISA analysis of V3S-0622-YC1902 was performed by coating with Recombinant Human IL1 Beta.
Figure 4 Anti-IL1B Monoclonal Antibody (V3S-0622-YC1902) in ELISA
