Anti-IL1B Neutralizing Antibody (V3S-0622-YC1902) (CAT#: V3S-0622-YC1902)

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  • fig1
    Figure 1 Anti-IL1B Monoclonal Antibody (V3S-0622-YC1902) in SDS-PAGE
  • fig1
    Figure 2 Anti-IL1B Monoclonal Antibody (V3S-0622-YC1902) in SEC-HPLC
  • fig1
    Figure 3 Anti-IL1B Monoclonal Antibody (V3S-0622-YC1902) in WB
  • fig1
    Figure 4 Anti-IL1B Monoclonal Antibody (V3S-0622-YC1902) in ELISA

Datasheet

MSDS

COA

Summary
Property
Applications
Protocols
Target

Summary

Description This product is a human monoclonal antibody that reacts with IL1B. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation.
Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality Monoclonal
Host Species Human
Target Species Human
Immunogen Recombinant human ILI beta
Isotype IgG

Property

Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.

Applications

Application WB; ELISA; IP; FC; FuncS; Neut; IF
Application Notes The antibody is recommended for detection of IL1B by WB, ELISA, IP, FC, FuncS, Neut, IF assays.

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target IL1B
Alternative Name Hematopoietin 1; IFN beta inducing factor; IL 1; IL 1 beta; IL 1B; IL-1 beta; IL1 BETA; IL1F2; Interleukin 1 beta; Interleukin 1 beta precursor; Interleukin-1 beta; LAF; OAF; Osteoclast activating factor; OTTHUM
Gene ID 3553
Research Area Cardiovascular

Tested Data

SDS-PAGE

Figure 1 Anti-IL1B Monoclonal Antibody (V3S-0622-YC1902) in SDS-PAGE

SDS-PAGE analysis of V3S-0622-YC1902 in non-reduced (lane 1) and β-mercaptoethanol-reduced (lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.

Figure 1 Anti-IL1B Monoclonal Antibody (V3S-0622-YC1902) in SDS-PAGE

SEC-HPLC

Figure 2 Anti-IL1B Monoclonal Antibody (V3S-0622-YC1902) in SEC-HPLC

The purity of V3S-0622-YC1902 was greater than 95% as determined by SEC-HPLC.

Figure 2 Anti-IL1B Monoclonal Antibody (V3S-0622-YC1902) in SEC-HPLC

WB

Figure 3 Anti-IL1B Monoclonal Antibody (V3S-0622-YC1902) in WB

Western blot analysis of V3S-0622-YC1902 was performed by loading Recombinant Human IL1 Beta.

Lane 1: Non-Reducing Antigen (1 μg)
Lane 2: Reducing Antigen (1 μg)

Figure 3 Anti-IL1B Monoclonal Antibody (V3S-0622-YC1902) in WB

ELISA

Figure 4 Anti-IL1B Monoclonal Antibody (V3S-0622-YC1902) in ELISA

ELISA analysis of V3S-0622-YC1902 was performed by coating with Recombinant Human IL1 Beta.

Figure 4 Anti-IL1B Monoclonal Antibody (V3S-0622-YC1902) in ELISA

For research use only, not directly for clinical use.
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