Anti-IL6 Neutralizing Antibody (V3S-0622-YC4771) (CAT#: V3S-0622-YC4771)

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  • fig1
    Figure 1 Anti-Human IL6 Monoclonal Antibody (V3S-0622-YC4771) in ELISA
  • fig1
    Figure 2 Anti-Human IL6 Monoclonal Antibody (V3S-0622-YC4771) in WB
  • fig1
    Figure 3 Anti-Human IL6 Monoclonal Antibody (V3S-0622-YC4771) in DB

Datasheet

MSDS

COA

Summary
Property
Applications
Protocols
Target

Summary

Description This product is a human monoclonal antibody that reacts with IL6. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation.
Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality Monoclonal
Host Species Human
Target Species Human
Immunogen Recombinant human IL-6.
Isotype IgG

Property

Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.

Applications

Application WB; DB; IF; IP; Neut; FuncS; ELISA; FC; ICC
Application Notes The antibody is recommended for detection of IL6 by IF, IP, Neut, FuncS, ELISA, FC, ICC assays.

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target IL6
Alternative Name IL6; interleukin 6; HGF; HSF; BSF2; IL-6; IFNB2; interleukin-6; CDF; BSF-2; IFN-beta-2; interferon beta-2; interleukin BSF-2; interferon, beta 2; hybridoma growth factor; CTL differentiation factor; B-cell stimulatory factor 2; B-cell differentiation factor
Gene ID 3569
UniProt B4DVM1
Research Area Immunology; Neuroscience

Tested Data

ELISA

Figure 1 Anti-Human IL6 Monoclonal Antibody (V3S-0622-YC4771) in ELISA

ELISA analysis of V3S-0622-YC4771 was performed by coating with human IL6 protein (His tag). Then blocked with BSA and incubated with V3S-0622-YC4771. The HRP-conjugated goat anti-IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 1 Anti-Human IL6 Monoclonal Antibody (V3S-0622-YC4771) in ELISA

WB

Figure 2 Anti-Human IL6 Monoclonal Antibody (V3S-0622-YC4771) in WB

Western blot analysis of V3S-0622-YC4771 was performed by loading human IL6 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0622-YC4771 and HRP goat anti-IgG (H+L) as a secondary antibody (1: 3000). Chemiluminescent detection was performed.

Figure 2 Anti-Human IL6 Monoclonal Antibody (V3S-0622-YC4771) in WB

DB

Figure 3 Anti-Human IL6 Monoclonal Antibody (V3S-0622-YC4771) in DB

Dot Blot analysis of V3S-0622-YC4771 was performed by coating with human IL6 protein (His tag).

V3S-0622-YC4771 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-IgG (H+L) as a secondary antibody (1: 3000)
NC: Negative control, PC: Positive control

Figure 3 Anti-Human IL6 Monoclonal Antibody (V3S-0622-YC4771) in DB

For research use only, not directly for clinical use.
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