| Description | This product is a monoclonal antibody derived from Rabbit (Oryctolagus cuniculus), which can specifically recognize Interleukin 6. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Rabbit |
| Target Species | Human, Cynomolgus, Mouse, Rat |
| Immunogen | huIL-6 |
| Affinity | KD = 4 pM (Human IL6), 31 pM (Cynomolgus IL6), 0.4 nM (Mouse IL6), 1.4 nM (Rat IL6), determined by surface plasmon resonance. |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | WB; DB; ELISA; Block; FuncS |
| Application Notes | The antibody is recommended for detection of IL6 by ELISA, Block, FuncS assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Rabbit Anti-IL6 Monoclonal Antibody (V3S-0522-YC2473) in ELISA
ELISA analysis of V3S-0522-YC2473 was performed by coating with human IL6 protein (His tag). Then blocked with BSA and incubated with V3S-0522-YC2473. The HRP-conjugated goat anti-IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 1 Rabbit Anti-IL6 Monoclonal Antibody (V3S-0522-YC2473) in ELISA
Figure 2 Rabbit Anti-IL6 Monoclonal Antibody (V3S-0522-YC2473) in WB
Western blot analysis of V3S-0522-YC2473 was performed by loading human IL6 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0522-YC2473 and HRP goat anti-IgG (H+L) as a secondary antibody (1: 3000). Chemiluminescent detection was performed.
Figure 2 Rabbit Anti-IL6 Monoclonal Antibody (V3S-0522-YC2473) in WB
Figure 3 Rabbit Anti-IL6 Monoclonal Antibody (V3S-0522-YC2473) in DB
Dot Blot analysis of V3S-0522-YC2473 was performed by coating with human IL6 protein (His tag).
V3S-0522-YC2473 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-IgG (H+L) as a secondary antibody (1: 3000)
NC: Negative control, PC: Positive control
Figure 3 Rabbit Anti-IL6 Monoclonal Antibody (V3S-0522-YC2473) in DB
