| Description | This product is a human antibody recognizing influenza A virus NA, termed as 1G04. It binds with multiple different influenza A and B virus neuraminidases and inhibits N2, N3 and certain N1 NAs. It shows some cross-reactivity to N3 and N6 (group 2), and N1, N5 and N8 (group 1), as well as weak binding to influenza B NA. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | IAV (N1, N2, N3, N5, N6 and N8) |
| Cross Reactivity | IBV |
| Epitope | Enzyme active site |
| IC50 | 0.1-10 μg/ml (group 2) |
| Affinity | 2.76 nM |
| Isotype | Human IgG |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; BLI; Neut; ELLA; MNA; ADCC |
| Application Notes | ELISA: The mAb was diluted to a starting concentration of 30 μg/mL, serially diluted 1:3, and incubated for 2 h at room temperature. Bio-layer interferometry (BLI): Each of the eight sensors were submerged into a different dilution of antibody (starting at 10 μg/mL and serially diluted two-fold) and measured for 300 seconds. Neutralization assay (Neut): The mAb was diluted to a starting concentration of 30 μg/mL, serially diluted 1:2 in UltraMDCK media. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | Influenza NA |
| Alternative Name | Neuraminidase |
| Research Area | Infectious Disease |
| Related Disease | Influenza |
