| Description | JUN1 is a neutralizing antibody targeting the GP1 of JUNV (Junín virus), derived from mouse. JUN1 exhibits neutralizing potency against HIV-1-based virus particles. It also neutralize in an immunofocus reduction neutralization assay, which is based on the envelope-chimeric LCMV used for live-attenuated immunization. This product has been detected using Enzyme-linked immunosorbent assay and Neutralization assay. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Junín virus (JUNV) |
| Epitope | JUN1 binds to JUNV GP1 at a site overlapping that used for TfR1 recognition. |
| Neutralization Mechanism | JUN1 binds the JUNV GP1 RBS by insertion of a tyrosine residue into the central pocket of the GP1, an interaction that mimics TfR1-NW GP1 recognition. |
| IC50 | 0.001 - 0.003 μg/mL |
| Isotype | Mouse IgG |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein G purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; Neut |
| Application Notes | ELISA: Serially diluted Ab (starting at 10 mg/mL, 1:5 dilution in blocking buffer) was added for 2 h at room temperature. Neutralization assay (Neut): Serial dilutions of MAb was prepared with DMEM and incubated with JUNV GP pseudotyped HIV-1 virus particles for 1 h at 37°C in 96-well plates. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | JUNV GP1 |
| Alternative Name | Junín virus glycoprotein complex |
| Research Area | Infectious Disease |
| Related Disease | Severe hemorrhagic fever |
Figure 1 JUNV GP1 (RBS) Specific Neutra™ Antibody Fab Fragment (V3S-0923-XY42) in Western Blot.
Western blot analysis of V3S-0923-XY42 was performed by loading recombinant reverse transcriptase onto a 12% Tris-HCl polyacrylamide gel. proteins were transferred to a NC membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0923-XY42 and HRP conjugated anti-mouse IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (1.5 μg)
Lane 2: Reduced antigen (1.0 μg)
Lane 3: Reduced antigen (0.5 μg)
Figure 1 JUNV GP1 (RBS) Specific Neutra™ Antibody Fab Fragment (V3S-0923-XY42) in Western Blot.
Figure 2 JUNV GP1 (RBS) Specific Neutra™ Antibody Fab Fragment (V3S-0923-XY42) in ELISA.
ELISA analysis of V3S-0923-XY42 was performed by coating with recombinant reverse transcriptase. Then blocked with BSA and incubated with V3S-0923-XY42. The HRP conjugated anti-mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 2 JUNV GP1 (RBS) Specific Neutra™ Antibody Fab Fragment (V3S-0923-XY42) in ELISA.
