JUNV GP1 (RBS) Specific Neutra™ Antibody (V3S-0923-XY42), Mouse IgG (CAT#: V3S-0923-XY42)

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  • fig1
    Figure 1 JUNV GP1 (RBS) Specific Neutra™ Antibody Fab Fragment (V3S-0923-XY42) in Western Blot.
  • fig1
    Figure 2 JUNV GP1 (RBS) Specific Neutra™ Antibody Fab Fragment (V3S-0923-XY42) in ELISA.

Datasheet

MSDS

COA

Summary
Property
Applications
Protocols
Target

Summary

Description JUN1 is a neutralizing antibody targeting the GP1 of JUNV (Junín virus), derived from mouse. JUN1 exhibits neutralizing potency against HIV-1-based virus particles. It also neutralize in an immunofocus reduction neutralization assay, which is based on the envelope-chimeric LCMV used for live-attenuated immunization. This product has been detected using Enzyme-linked immunosorbent assay and Neutralization assay.
Clonality Monoclonal
Host Species Mouse
Target Species Junín virus (JUNV)
Epitope JUN1 binds to JUNV GP1 at a site overlapping that used for TfR1 recognition.
Neutralization Mechanism JUN1 binds the JUNV GP1 RBS by insertion of a tyrosine residue into the central pocket of the GP1, an interaction that mimics TfR1-NW GP1 recognition.
IC50 0.001 - 0.003 μg/mL
Isotype Mouse IgG

Property

Expression Species HEK293F or CHO cell line
Conjugation Unconjugated
Purity >95%
Endotoxin <1 EU/mg
Form Liquid
Purification Protein G purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics.

Applications

Application ELISA; Neut
Application Notes ELISA: Serially diluted Ab (starting at 10 mg/mL, 1:5 dilution in blocking buffer) was added for 2 h at room temperature.
Neutralization assay (Neut): Serial dilutions of MAb was prepared with DMEM and incubated with JUNV GP pseudotyped HIV-1 virus particles for 1 h at 37°C in 96-well plates.

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target JUNV GP1
Alternative Name Junín virus glycoprotein complex
Research Area Infectious Disease
Related Disease Severe hemorrhagic fever

Tested Data

WB

Figure 1 JUNV GP1 (RBS) Specific Neutra™ Antibody Fab Fragment (V3S-0923-XY42) in Western Blot.

Western blot analysis of V3S-0923-XY42 was performed by loading recombinant reverse transcriptase onto a 12% Tris-HCl polyacrylamide gel. proteins were transferred to a NC membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with V3S-0923-XY42 and HRP conjugated anti-mouse IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.

Lane 1: Reduced antigen (1.5 μg)
Lane 2: Reduced antigen (1.0 μg)
Lane 3: Reduced antigen (0.5 μg)

Figure 1 JUNV GP1 (RBS) Specific Neutra™ Antibody Fab Fragment (V3S-0923-XY42) in Western Blot.

ELISA

Figure 2 JUNV GP1 (RBS) Specific Neutra™ Antibody Fab Fragment (V3S-0923-XY42) in ELISA.

ELISA analysis of V3S-0923-XY42 was performed by coating with recombinant reverse transcriptase. Then blocked with BSA and incubated with V3S-0923-XY42. The HRP conjugated anti-mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 2 JUNV GP1 (RBS) Specific Neutra™ Antibody Fab Fragment (V3S-0923-XY42) in ELISA.

For research use only, not directly for clinical use.
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