| Description | This product is a human monoclonal antibody that reacts with LRRC15. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; WB |
| Application Notes | The antibody is recommended for detection of LRRC15 by ELISA, WB assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | LRRC15 |
| Alternative Name | leucine rich repeat containing 15; LIB; leucine-rich repeat-containing protein 15; hLib; leucine-rich repeat protein induced by beta amyloid; leucine-rich repeat protein induced by beta-amyloid homolog |
| Gene ID | 131578 |
| UniProt | Q8TF66 |
| Research Area | Neuroscience; Cancer Research |
Figure 1 Afuco™ Recombinant Anti-LRRC15 Antibody (V3S-0622-YC3637), ADCC Enhanced, in SEC-HPLC.
The purity of V3S-0622-YC3637 was 97.1% as determined by SEC-HPLC.
Figure 1 Afuco™ Recombinant Anti-LRRC15 Antibody (V3S-0622-YC3637), ADCC Enhanced, in SEC-HPLC.
Figure 2 Afuco™ Recombinant Anti-LRRC15 Antibody (V3S-0622-YC3637), ADCC Enhanced, in ELISA.
ELISA analysis of V3S-0622-YC3637 was performed by coating with recombinant LRRC15 protein. Then blocked with BSA and incubated with the antibody. The goat anti-human IgG-HRP as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 2 Afuco™ Recombinant Anti-LRRC15 Antibody (V3S-0622-YC3637), ADCC Enhanced, in ELISA.
