| Description | This mouse neutralizing antibody is specific for MACV (Machupo virus) GP, termed as MAC1. It targets an overlapping binding site at the rim of the central TfR1-binding pocket that includes MACV GP1 loops 3, 7, and 10. The antibody has potent neutralizing ability against MACV. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Machupo virus (MACV) |
| Epitope | MAC1 targets an overlapping binding site at the rim of the central TfR1-binding pocket that includes MACV GP1 loops 3, 7, and 10. |
| Neutralization Mechanism | The antibody neutralizes through disruption of hTfR1 recognition |
| IC50 | 0.004 μg/mL |
| Isotype | Mouse IgG |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein G purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; Neut |
| Application Notes | ELISA: Serially diluted Ab (starting at 10 mg/mL, 1:5 dilution in blocking buffer) was added for 2 h at room temperature. Neutralization assay (Neut): Serial dilutions of MAbs were prepared with DMEM and incubated with MACV GP pseudotyped HIV-1 virus particles for 1 h at 37°C in 96-well plates. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | MACV GP1 |
| Alternative Name | Machupo virus glycoprotein |
| Research Area | Infectious Disease |
| Related Disease | Severe hemorrhagic fever |
