Recombinant Anti-MCAM Antibody (V3S-0622-YC3664) (CAT#: V3S-0622-YC3664)

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Datasheet

MSDS

COA

Sub Cat Isotype Application Affinity  
V3S-1124-YC515 IgG1 kappa ELISA; FC Inquiry
V3S-0622-YC4909 IgG1 FC; WB; IHC Inquiry
V3S-0622-YC4910 IgG3 FC; WB; IHC Inquiry
V3S-0622-YC4913 IgG ELISA Inquiry
V3S-1022-YC2286 IgG1 IP; FC Inquiry
V3S-1022-YC2287 IgG1 kappa IP; FC Inquiry
V3S-0622-YC4907 IgG FuncS Inquiry
V3S-0723-FY221 IgG1 kappa FC; ELISA; Block 0.221 nM Inquiry
Summary
Property
Applications
Protocols
Target

Summary

Description This product is a human monoclonal antibody that reacts with MCAM. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation.
Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality Monoclonal
Host Species Human
Target Species Human
Isotype IgG1 kappa

Property

Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.

Applications

Application ELISA; FC
Application Notes The antibody is recommended for detection of MCAM by ELISA, FC assays.

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target MCAM
Alternative Name MCAM; Melanoma-Associated Antigen MUC18; Cell Surface Glycoprotein P1H12; CD146; S-Endo 1 Endothelial-Associated Antigen; Gicerin; MUC18; Melanoma-Associated Antigen A32
Gene ID 4162
UniProt P43121
Research Area Immunology; Signal Pathway
For research use only, not directly for clinical use.
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