| Description | The antibody is a complement-activating, bactericidal monoclonal antibody that targets the outer membrane protein PorA epitope P1.16 of N. meningitidis. Its binding to PorA on the meningococcal surface activates the classical complement pathway, leading to bacterial lysis. This antibody is elicited against loop 4 of PorA, which serves as a model for studying the molecular and structural details of bactericidal antibody recognition of PorA. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Neisseria meningitidis |
| Immunogen | N. meningitidis outer membrane protein PorA |
| Epitope | An epitope containing the sequence TKDTNNNL. |
| Isotype | IgG2a, κ |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | WB; ELISA; Neut |
| Application Notes | The antibody is recommended for detection of N. meningitidis porA by WB (1/1000-1/5000), ELISA, Neut assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
