| Description | F018 is a fully human antibody that exhibits specific binding to nicotine. The dissociation constants (KD) for the antibody's interactions with (R)-(+)-nicotine, as determined by equilibrium dialysis, are measured at 3.1 nM for clone F018. The isotype is IgG2. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Immunogen | Amplification of a DNA sequence library encoding the human kappa light chain variable region from cDNA from B cells of human subjects immunized with nicotine vector conjugates. |
| Affinity | KD = 3.1 nM |
| Isotype | Human IgG2, lambda |
| Expression Species | HEK293F or CHO |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; Inhib |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | Nicotine |
| Alternative Name | Nicotine |
| Related pathway | ERK1/2/MAPK p38/JNK and PI3K/AKT pathways |
| Research Area | Chemical; Signal Pathway |
