| Description | This product is a monoclonal antibody derived from Human (Homo sapiens), which can specifically recognize Plasminogen activator, urokinase receptor. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; FC; WB; IP |
| Application Notes | The antibody is recommended for detection of PLAUR by ELISA, FC, WB, IP assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | PLAUR |
| Alternative Name | PLAUR; Plasminogen activator, urokinase receptor; Urokinase receptor; uPA receptor; uPAR; CD87; Cluster of Differentiation 87; Urokinase Plasminogen Activator Receptor; UPAR; URKR; U-PAR |
| Gene ID | 5329 |
| UniProt | Q03405 |
| Research Area | Cardiovascular |
| Related Disease | Breast cancer |
Figure 1 Anti-PLAUR Monoclonal Antibody (V3S-0522-YC1246) in SDS-PAGE
SDS-PAGE analysis of V3S-0522-YC1246 in non-reduced and β-mercaptoethanol-reduced conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 30 kDa, respectively. And non-reduced protein migrates as 175 kDa.
Figure 1 Anti-PLAUR Monoclonal Antibody (V3S-0522-YC1246) in SDS-PAGE
Figure 2 Anti-PLAUR Monoclonal Antibody (V3S-0522-YC1246) in SEC-HPLC
The purity of V3S-0522-YC1246 was greater than 95% as determined by SEC-HPLC.
Figure 2 Anti-PLAUR Monoclonal Antibody (V3S-0522-YC1246) in SEC-HPLC
Figure 3 Anti-PLAUR Monoclonal Antibody (V3S-0522-YC1246) in ELISA
ELISA analysis of V3S-0522-YC1246was performed by coating with Recombinant Human PLAUR Protein. Then blocked with BSA, and incubated with Anti-Human PLAUR Antibody (V3S-0522-YC1246). The HRP-conjugated goat anti-Mouse IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 3 Anti-PLAUR Monoclonal Antibody (V3S-0522-YC1246) in ELISA
