Anti-RSPO3 Neutralizing Antibody (V3S-0522-YC410) (CAT#: V3S-0522-YC410)

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Datasheet

MSDS

COA

Sub Cat Target Species Isotype Application  
V3S-1124-YC742 Human, Mouse IgG1 kappa ELISA; Inhib Inquiry
V3S-0522-YC411 Human IgG2 kappa ELISA; Inhib Inquiry
V3S-0522-YC1475 Human IgG1 kappa ELISA; IHC; FC; IP; IF; FuncS Inquiry
V3S-1022-YC467 Human IgG Inhib Inquiry
Summary
Property
Applications
Protocols
Target

Summary

Description This product is a monoclonal antibody derived from Human (Homo sapiens), which can specifically recognize R-spondin 3. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation.
Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free.
Clonality Monoclonal
Host Species Human
Target Species Human, Mouse
Isotype IgG1, κ

Property

Expression Species HEK293F or CHO
Conjugation None
Purity >95%, determined by SDS-PAGE and/or SEC-HPLC
Endotoxin <1 EU/mg, determined by LAL method
Purification Protein A affinity purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C.

Applications

Application ELISA; Inhib
Application Notes The antibody is recommended for detection of RSPO3 by ELISA, Inhib assays.

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target RSPO3
Alternative Name RSPO3; R-spondin 3; PWTSR; THSD2; CRISTIN1; R-spondin-3; R-spondin 3 homolog; roof plate-specific spondin-3; protein with TSP type-1 repeat; thrombospondin; type I; domain containing 2; thrombospondin type-1 domain-containing protein 2; anti-RSPO3
Gene ID 84870
UniProt Q9BXY4
Research Area Stem Cell
Related Disease Non-small cell lung tumor
For research use only, not directly for clinical use.
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