| Description | The antibody BG4-25 is a human mAb specific for SARS-CoV-2 Spike S1 protein. It shows exceptional neutralizing activity. It recognizes an RBD epitope that overlaps with>90% of residues in the ACE2 RBM, which is only fully accessible with "up" RBD conformations. The IC50 of antibody is 4 ng/mL. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | SARS-CoV-2 |
| Epitope | Overlaps with>90% of residues in the ACE2 RBM |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA, Neut |
| Application Notes | The IC50 of BG4-25 is 0.004 μg/mL. MaxiSorp 384-well micro-plates were coated with 50 μl/well of 2,500 ng/ml of SARS-CoV-2 RBD in coating buffer overnight at 4°C. Plates were then washed 3 times with 100 μl/well of wash buffer using a BioTek 406 plate washer. Plates were blocked by adding 100 μl/well of blocking buffer for 30 min at room temperature. Plates were then washed as described above. 50 μL of 1:100 diluted serum samples in dilution buffer were added to the wells and incubated for 30 min at 37°C. Plates were then washed 7 times as described above. 50 μl/well of 1:25,000 diluted detection Ab solution was added to the wells and incubated for 30 min at room temperature. Plates were then washed 7 times as described above. 40 μl/well of Pierce TMB peroxidase substrate was then added to the wells and incubated at room temperature for 3 min or 5 min. The reaction was then stopped by adding 40 μl/well of stop solution to each well. The OD was read after 15 min at 450 nm and 570 nm on a BioTek Synergy HT using Gen5 software. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Western Blot Protocol |
| Target | SARS-CoV-2 |
| Alternative Name | Severe acute respiratory syndrome coronavirus 2 |
| Research Area | Coronavirus Disease 2019 |
| Related Disease | Coronavirus Disease 2019 |
