| Description | The antibody GAR12 is a human mAb specific for SARS-CoV-2 Spike S1 protein. It is particularly broad in specificity and neutralized all analyzed variants of concern, with antibody neutralizing with IC50s ranging from 255 to 128 ng/ml. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | SARS-CoV-2 |
| Epitope | Receptor-binding domain (RBD) |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA, Neut |
| Application Notes | The IC50 value of GAR12 is ranging from 255 to 128 ng/ml. Maxisorp plates were coated with SARS-CoV-2 RBD at 2 µg/ml in carbonate coating buffer overnight at 4°C. The following day, plates were washed twice with PBS and blocked in 4% milk in PBS-T for 2 h. Plates were washed 3x with PBS-T and incubated with the antibody GAR12 diluted to 100 µg/ml in PBS for 1 h. Plates were then washed 3x with PBS-T and incubated with HRP-conjugated goat Anti-human IgG antibody for 1 h. Plates were finally washed 2x with PBS-T and 1x with PBS and incubated with TMB substrate until the reaction was quenched with HCl. Plates were read at Abs 450 nm. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Western Blot Protocol |
| Target | SARS-CoV-2 |
| Alternative Name | Severe acute respiratory syndrome coronavirus 2 |
| Research Area | Coronavirus Disease 2019 |
| Related Disease | Coronavirus Disease 2019 |
